Tools - Based Search for Potential Human Wheat Germ Agglutinin - Immunoreactive
نویسنده
چکیده
Following the initial observation about the exsistence of plant lectins-immunoreactive material in different rat and human tissues (D ebb age et al. 1993), we isolated a specific wheat germ agglutinin (WGA)-immunoreactive (IR) protein from human placenta by combination of ion-exchange and immunoaffinity chromatography. It was characterized as 66 kD glycoprotein exhibiting calcium-dependent, carbohydratebinding activity (J a n k 0 vic and Vic 0 v a c 2000). Placental WGA-IR protein was distributed at syncityotrophoblast brush border and cytotrophoblast of first trimester human placenta and the immunoreactivity was also found in choriocarcinoma cell lines. In addition to this, the protein with the same biochemical characteristics was isolated from human normal and pregnancy sera (J a n k 0 vic 2000).
منابع مشابه
An Alkaline Phosphatase Lacking Wheat Germ Agglutinin Binding Sites Useful Enzyme for Lectin Assays with Comparable Activity to the Calf Enzyme
Despite the availability of various alkaline phosphatase (ALP) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. The glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (WGA). As a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...
متن کاملIncrease in cell surface wheat germ agglutinin binding in a rat hepatoma cell line dRLa 74 treated with concanavalin A.
Wheat germ agglutinin binding to a rat hepatoma cell line dRLa 74 treated with concanavalin A was studied. It increased depending on the concanavalin A concentration in the culture medium. The cells exhibited about twofold increase in wheat germ agglutinin-binding when pretreated with 50 micrograms/ml of concanavalin A for 48 h. The wheat germ agglutinin binding sites were shown to be localized...
متن کاملInteraction of sialoglycoproteins with wheat germ agglutinin-sepharose of varying ratio of lectin to Sepharose. Use for the purification of mucin glycoproteins from membrane extracts.
The influence of varying the amount of wheat germ agglutinin immobilized on Sepharose beads on the binding of glycoproteins to these beads was investigated. A series of wheat germ agglutinin-Sepharose gels containing between 0.10 and 10.0 mg of lectin/ml of gel was prepared, and the actual lectin content was established by acid hydrolysis of the gel followed by analysis of glycine, a major amin...
متن کاملSelective inhibition of a step of myotube formation with wheat germ agglutinin in a murine myoblast cell line, C2C12.
Myoblast cells, C2C12, which is an established cell line from satellite cells of skeletal muscle of C3H mouse, start to fuse and form multinucleated cells (myotubes) and begin to express creatine phosphokinase and myosin, when culture medium is changed from the growth medium to the differentiation medium. Among the 12 lectins that we tested, wheat germ agglutinin apparently suppressed the myotu...
متن کاملIn Vitro Antitrichomonal Effect of Nigella Sativa Aqueous Extract and Wheat Germ Agglutinin
Trichomoniasis is one of the most common parasitic sexually transmitted diseases in the world. Metronidazole was known as the most effective drug for human trichomoniasis., however, drug resistance and toxicity appeared. This study was designed to investigate the in vitro inhibitory activity of wheat germ agglutinin and Nigella sativa aqueous extract on the growth and motility of Trichomonas va...
متن کامل